Rapid Detection of Salmonella Microcolonies by Fluorescent Antibody

Visualization of Horse Sickness virus by the Fluorescent antibody technique

Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies.

CD46 (membrane cofactor protein), a complement-regulatory protein that participates in innate and acquired immunity, also serves as a receptor for viral and bacterial pathogens. CD46 isoforms terminate in one of two cytoplasmic tails, Cyt1 or Cyt2, which differ in signaling and trafficking properties. Dissecting the functions of the two cytoplasmic tails in these cellular processes has been ham...

rapid and specific detection of salmonella typhimurium by pcr-elisa

objectives: salmonella typhimurium is important food-borne pathogen responsible for gastroenteritis. in this work a polymerase chain reaction based enzyme linked immunosorbent assay (pcr-elisa) was developed to identify salmonella typhimurium. materials and methods: the rfb gene which is responsible for biosynthesis of the salmonella o-antigenic lipopolysaccharide was selected as the target s...

Rapid fluorescent-antibody staining technique.

The rapid fluorescent-antibody staining technique described by Kellogg and Deacon for staining Neisseria gonorrhoeae and Treponema pallidum was applied to fluorescent-antibody tests for group A streptococci and enteropathogenic Escherichia coli. Results obtained with this staining technique were compared with results using the conventional staining procedure; excellent correlation was obtained....

Rapid fluorescent-antibody conjugation procedure.

This note describes a technique developed for rapid preparation of specific and brilliant conjugates for direct staining of rickettsial agents in yolk-sac, tick, and vertebrate tissues.